Immunostaining of COX-2 in Model of Breast Carcinogenesis by Chemical Induction

Abstract

The breast cancer is one of the main organs of the human body which are not fully developed after birth, develops due lifelong through different modifications. It is known that this body mutates and can develop a breast cancer which is the most diagnosed cancer worldwide among women being responsible for the high mortality rate among women. This neoplasm depends on various biological and molecular factors where there is evidence of COX-2 expression in breast tumors, with difficulties in therapeutic strategies promoting the importance of studying this malignancy. This paper aims to, through methodology, to assess Cox-2 expression in breast carcinoma models generated by chemical induction with DMBA in virgin Sprague-Dawley rats 40 to 45 days of life in a dose 100 mg / kg of body weight diluted in 1 ml of soybean oil and administered intragastrically by gavage. Accordance with this method yielded average results of 4 tumors developed by these animals being found bilaterally. The mean tumor volume was 3,74cm 3 yielding predominant histological subtypes ductal carcinoma, phyllodes tumors and carcinomas papillary. The immunostaining of COX-2 was predominantly cytoplasmic and nuclear, manifesting only in ductal carcinoma tissue and is expressed in 55.61% of the cancer cells, finding that the mammary carcinogenesis model by DMBA in virgin SD rats are a good model to be used in experimental studies in vivo focused on COX-2.