Determination of calves’ levels of lactate coming from somatic cell nuclear transfer technique

Abstract

Cattle raising is responsible for the vast majority of clones produced for commercial purposes. Currently, there are more than 1500 animals, which are born by SCNT worldwide. In Brazil, 109 clones were catalogued until 2014. Biochemical profile is valuable for body score condition assessment, once high levels of lactate at birth is an indicator of hypoxia – metabolic condition capable of damaging a newborn’s Central Nervous System and Sympathetic Nervous System. Measuring plasmatic lactate levels is a manner of determining tissue hypoxia in newborns. This study aims at assessing lactate levels in newborns at birth and 48 hours later, in order to correlate the anylite and the occurrence of
respiratory acidosis. Twenty four samples of peripheral blood have been taken from twelve Gyr, Nelore, and Senepol cows between 0 – 48 hours after birth. Analyses were carried out in a TEKNA 3000 VET Biochemistry Analyzer Automatic. Average lactate levels were 116,5 mg/dL at 0 hour and 75,5 mg/dL at 48 hours. Some analyzed cases showed significates difference when compared with the literature, that’s demonstrate the important of this biochemistry marker, specialty in the first hour calve’s life.